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Promega 5× reaction buffer m289a
5× Reaction Buffer M289a, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/5+%C2%B7+reaction+buffer/5%C3%97+reaction+buffer+promega+m289a/pm40251891-71-13-16
Average 90 stars, based on 1 article reviews
5× reaction buffer m289a - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Centrifugation:

Article Title: Opposing effects of phorbol-12-myristate-13-acetate, an activator of protein kinase C, on the signaling of structurally related human dopamine D1 and D5 receptors.
Article Snippet: .. Following a 1 min centrifugation at 10 000 g, the supernatant was carefully removed to a fresh tube and the RNA was used immediately or stored at )70 C. The RNA (3 lg) was reverse transcribed in a 50 lL reaction by incubation with avian myeloblastosis virus-reverse transcriptase (15 U, Promega, Madison, WI, USA), 5 · reaction buffer (Promega), dNTPs (1 mM, Roche, Indianapolis, IN, USA), Rnasin (50 U, Promega) and random hexamers (0.108 A260 U, Roche), at 42 C for 30 min to 1 h. As controls for each RNA sample, parallel reactions were run with no reverse transcriptase and another with no RNA. .. Reverse transcription reactions (4 lL) were then subjected to PCR in a 50 lL volume containing, 10 · reaction buffer, dNTPs (0.2 mM, MBI Fermentas inc., Burlington, ON, Canada), MgCl2 (1.5 mM, Invitrogen), specific human PKC isoform or glyceraldehyde-3phosphate dehydrogenase (GAPDH) primers (taken from Webb et al. 1997) (0.06–0.25 lM, see Table 1 for primer sequences and GenBank accession numbers) and Taq DNA polymerase (2.5 U, Invitrogen).

Reverse Transcription:

Article Title: Opposing effects of phorbol-12-myristate-13-acetate, an activator of protein kinase C, on the signaling of structurally related human dopamine D1 and D5 receptors.
Article Snippet: .. Following a 1 min centrifugation at 10 000 g, the supernatant was carefully removed to a fresh tube and the RNA was used immediately or stored at )70 C. The RNA (3 lg) was reverse transcribed in a 50 lL reaction by incubation with avian myeloblastosis virus-reverse transcriptase (15 U, Promega, Madison, WI, USA), 5 · reaction buffer (Promega), dNTPs (1 mM, Roche, Indianapolis, IN, USA), Rnasin (50 U, Promega) and random hexamers (0.108 A260 U, Roche), at 42 C for 30 min to 1 h. As controls for each RNA sample, parallel reactions were run with no reverse transcriptase and another with no RNA. .. Reverse transcription reactions (4 lL) were then subjected to PCR in a 50 lL volume containing, 10 · reaction buffer, dNTPs (0.2 mM, MBI Fermentas inc., Burlington, ON, Canada), MgCl2 (1.5 mM, Invitrogen), specific human PKC isoform or glyceraldehyde-3phosphate dehydrogenase (GAPDH) primers (taken from Webb et al. 1997) (0.06–0.25 lM, see Table 1 for primer sequences and GenBank accession numbers) and Taq DNA polymerase (2.5 U, Invitrogen).

Article Title: Insulin-Like Growth Factor 2 Promotes Osteogenic Cell Differentiation in the Parthenogenetic Murine Embryonic Stem Cells
Article Snippet: Embryonic stem cells (ESCs) are pluripotent and can differentiate into all somatic cell types.. ESCs are an alternative solution to hard tissue regeneration and skeletal tissue repair to treat bone diseases and defects using regenerative strategies.. Parthenogenetic ESCs (PESCs) may be a useful alternative stem cell source for tissue repair and regeneration.

Incubation:

Article Title: Opposing effects of phorbol-12-myristate-13-acetate, an activator of protein kinase C, on the signaling of structurally related human dopamine D1 and D5 receptors.
Article Snippet: .. Following a 1 min centrifugation at 10 000 g, the supernatant was carefully removed to a fresh tube and the RNA was used immediately or stored at )70 C. The RNA (3 lg) was reverse transcribed in a 50 lL reaction by incubation with avian myeloblastosis virus-reverse transcriptase (15 U, Promega, Madison, WI, USA), 5 · reaction buffer (Promega), dNTPs (1 mM, Roche, Indianapolis, IN, USA), Rnasin (50 U, Promega) and random hexamers (0.108 A260 U, Roche), at 42 C for 30 min to 1 h. As controls for each RNA sample, parallel reactions were run with no reverse transcriptase and another with no RNA. .. Reverse transcription reactions (4 lL) were then subjected to PCR in a 50 lL volume containing, 10 · reaction buffer, dNTPs (0.2 mM, MBI Fermentas inc., Burlington, ON, Canada), MgCl2 (1.5 mM, Invitrogen), specific human PKC isoform or glyceraldehyde-3phosphate dehydrogenase (GAPDH) primers (taken from Webb et al. 1997) (0.06–0.25 lM, see Table 1 for primer sequences and GenBank accession numbers) and Taq DNA polymerase (2.5 U, Invitrogen).

Virus:

Article Title: Opposing effects of phorbol-12-myristate-13-acetate, an activator of protein kinase C, on the signaling of structurally related human dopamine D1 and D5 receptors.
Article Snippet: .. Following a 1 min centrifugation at 10 000 g, the supernatant was carefully removed to a fresh tube and the RNA was used immediately or stored at )70 C. The RNA (3 lg) was reverse transcribed in a 50 lL reaction by incubation with avian myeloblastosis virus-reverse transcriptase (15 U, Promega, Madison, WI, USA), 5 · reaction buffer (Promega), dNTPs (1 mM, Roche, Indianapolis, IN, USA), Rnasin (50 U, Promega) and random hexamers (0.108 A260 U, Roche), at 42 C for 30 min to 1 h. As controls for each RNA sample, parallel reactions were run with no reverse transcriptase and another with no RNA. .. Reverse transcription reactions (4 lL) were then subjected to PCR in a 50 lL volume containing, 10 · reaction buffer, dNTPs (0.2 mM, MBI Fermentas inc., Burlington, ON, Canada), MgCl2 (1.5 mM, Invitrogen), specific human PKC isoform or glyceraldehyde-3phosphate dehydrogenase (GAPDH) primers (taken from Webb et al. 1997) (0.06–0.25 lM, see Table 1 for primer sequences and GenBank accession numbers) and Taq DNA polymerase (2.5 U, Invitrogen).



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